mouse anti drd1  (Santa Cruz Biotechnology)

Journal: Scientific reports

Article Title: GABA B receptor signaling in the caudate putamen is involved in binge-like consumption during a high fat diet in mice.

doi: 10.1038/s41598-021-98590-9

Figure Lengend Snippet: Figure 3. GABABR deficiency in striatum neurons increases expression of cFos + /drd1 + cells in CPu neurons. (A and C) Representative photographs showing the staining of cFos (green), dopamine receptor d1 (drd1) (red), and DAPI (blue) in the caudate putamen (CPu) (A) and nucleus accumbens (NAc) (C) of wild-type (WT) and knockout (KO) mice in the binge group. White arrowheads show the colocalization of cFos and drd1. Scale bars: 50 μm. (B and D) cFos-positive cells in drd1-positive neurons (cFos + /drd1 +) in the CPu (B) and NAc (D) of WT and KO mice in the binge group. All values are mean ± SEM. Statistical analyses were performed using two-way factorial analysis of variance (ANOVA) followed by Sidak’s post-hoc test (B and D). ns: not significant. #p < 0.05 versus 0 min in WT mice. †p < 0.05 versus 0 min in KO mice. *p < 0.05 versus WT mice. See also Supplemental Table 2 for details on statistics.

Article Snippet: They were then incubated in diluted blocking buffer (Blocking one, NACALAI TESQUE, Japan) and 0.2% sodium azide for 5 days at 4 °C with the following antibodies: rabbit anti-cFos (226 003, Synaptic Systems, Goettingen, Germany, RRID: AB_2231974), and mouse anti-drd1 (sc-33660, Santa Cruz Biotechnology, Dallas, TX, USA, RRID: AB_668813).

Techniques: Expressing, Staining, Knock-Out

Journal: Scientific reports

Article Title: GABA B receptor signaling in the caudate putamen is involved in binge-like consumption during a high fat diet in mice.

doi: 10.1038/s41598-021-98590-9

Figure Lengend Snippet: Figure 6. Suppressive effects of baclofen on cFos + /drd1 + cell expression in the CPu were absent in KO mice. (A and C) Representative photographs showing the staining of cFos (green), dopamine receptor d1 (drd1) (red), and DAPI (blue) in the caudate putamen (CPu) (A) and nucleus accumbens (NAc) (C) of wild-type (WT) and knockout (KO) mice at 30 min on a high fat diet (HFD) in the binge group. White arrowheads show the colocalization of cFos and drd1. Scale bars: 50 μm. (B and D) cFos-positive cells in drd1-positive neurons (cFos + /drd1 +) in the CPu (B) and NAc (D) of WT and KO mice at 30 min on a HFD in the binge group. All values are mean ± SEM. Statistical analyses were performed using two-way factorial analysis of variance (ANOVA) followed by Sidak’s post-hoc test (B and D). ns: not significant, veh: vehicle, bac: baclofen. *p < 0.05 versus vehicle in WT mice. See also Supplemental Table 2 for details on statistics.

Article Snippet: They were then incubated in diluted blocking buffer (Blocking one, NACALAI TESQUE, Japan) and 0.2% sodium azide for 5 days at 4 °C with the following antibodies: rabbit anti-cFos (226 003, Synaptic Systems, Goettingen, Germany, RRID: AB_2231974), and mouse anti-drd1 (sc-33660, Santa Cruz Biotechnology, Dallas, TX, USA, RRID: AB_668813).

Techniques: Expressing, Staining, Knock-Out

Journal: Scientific reports

Article Title: GABA B receptor signaling in the caudate putamen is involved in binge-like consumption during a high fat diet in mice.

doi: 10.1038/s41598-021-98590-9

Figure Lengend Snippet: Figure 7. Schematic of role of GABABR signaling in striatum in suppression of binge-like consumption of a HFD. We suggest that gamma-aminobutyric acid type B receptor (GABABR) signaling in the caudate putamen (CPu) suppresses the binge-like consumption of a high fat diet (HFD) by inhibiting downstream of dopamine receptor d1 (drd1) signaling in the CPu. The figure image was drawn using PowerPoint 2017 (Microsoft, USA).

Article Snippet: They were then incubated in diluted blocking buffer (Blocking one, NACALAI TESQUE, Japan) and 0.2% sodium azide for 5 days at 4 °C with the following antibodies: rabbit anti-cFos (226 003, Synaptic Systems, Goettingen, Germany, RRID: AB_2231974), and mouse anti-drd1 (sc-33660, Santa Cruz Biotechnology, Dallas, TX, USA, RRID: AB_668813).

Techniques: